Molecular Identification of Chlamydia trachomatis in Women with Recurrent Miscarriage with the Aim of Infection Control in Pregnancy

Background: Determination of the prevalence of Chlamydia trachomatis as an obligate intracellular bacterium, and one of the main causes of genital and sexually transmitted diseases, which is asymptomatic or associated with symptoms, can lead to a prenatal care system in the prevention of miscarriage. By identifying this abundance, planning can be made to achieve the control of recurrent abortion. Therefore, the main goal of this study is using the PCR molecular method to detect this bacterium in women with recurrent miscarriage. Methods: In this study, during April to December 2017, samples were collected using endo-cervical swab from a total of 100 women (with a history ofrecurrent miscarriage) who referred to infertility and perinatal clinics of Sarem Hospital. Samples were evaluated with two objectives: vaginal culture and PCR testing. DNA was extracted by using phenol-chloroform method. The PCR test was done for detection of Chlamydia trachomatis. Results: From a total of 100 vaginal samples, 14 cases (14%) were positive for chlamydia trachomatis. These positive samples also were reported positive in terms of bacterial infection by vaginal culture and patients had higher thannormal WBC count. & nbsp. Conclusion: Regarding the prevalence of chlamydial infection that is commonly found in the studied population, screening for pregnant women is recommended. Moreover, due to the advantages of PCR testing, because of high sensitivity and reduced response time, the use of molecular method for diagnosis of this bacterium in women with recurrent miscarriage has been proposed as a part of the country s health programs.  

کلیدواژه ها:

Molecular Identification ، Chlamydia trachomatis ، Recurrent miscarriage ، Infection Control