Ovarian Hyperstimulation Syndrome Model in Mice

Ovarian Hyperstimulation syndrome (OHSS) is a potentially life-threatening complication caused by ovarian stimulation during which the ovaries become severely stimulated. OHSS is induced by an ovarian release of angiogenic, vasoactive. The ovaries are swelling and being painful. Since the negative effects of OHSS are not really clear,therefore, an animal model is needed in order to evaluate OHSS effects on embryo. In this study we use Dana protocol to generate OHSS model in mice for first time in Iran. PMSG (20 IU/d) was administered ip to 6-week-old NMRI female mice for three subsequent days, followed by HCG administration (7 IU/d) on the fourth day. Mice were anesthetized 48 hours after HCG. Evans blue dye was injected Iv.After 30 minutes, 2 mL of sterile saline was injected ip, after which the abdominal cavity was opened and 1 mL of fluid centrifuged and pipetted into ELISA plates to record absorbance at 620 nm. 48 hours after HCG administration, we assessed body weight, ovarian weight; and vascular permeability to verify the OHSS model. Enhanced micro vascular permeability and its related edema that resulted in increased body weight along with ovary enlargement confirmed the OHSS model in mice OHSS is a potentially life-threatening complication in which affects people at risk especially young women and those who has a strong track record of polycystic ovarian disease or hypothyroidism to be taken hyper prolactinemia. Young women with polycystic ovary syndrome who have many follicles in the ovaries themselves are at high risk for ovarian Hyperstimulation syndrome.  

کلیدواژه ها:

Ovarian Hyper Stimulation ، Animal Model ، Mice