Insight to Collateral Testicular DNA Damage And/Or Apoptosis in Experimentally-Induced Left Side Varicocele

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 459

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شناسه ملی سند علمی:

ISERB04_163

تاریخ نمایه سازی: 16 تیر 1397

چکیده مقاله:

Background: Varicocele (VCL) is the most common multi factorial disorder in infertile men population. The VCL is characterized mainly by blood flow-down in spermatic and cremasteric veins in the left side testicles. Although various previous studies have focused on VCL-induced derangements in left side, the collateral effect of VCL especially apoptosis of germ cells has been remained unclear. Thus, the current study tried to illustrate the VCL-induced germ cells apoptosis as well as the cross -link between Proliferating-Cell-Nuclear-Antigen (PCNA) and DNA fragmentation in collateral testicular tissue Method and Material: To follow-up present study, 16 mature Wistar rats were divided into control -sham and left hadn side VCL-induced groups (NO=8 rats in each group). After 4 months, the collateral testicles were dissected out and the tubular differentiation (TDI), spermiogenesis indices (SPI) and histological alterations were investigated.Moreover, the Bcl-2+, Caspase-3+ and PCNA+ cells distribution was analyzed by using immunohistochemistry (IHC) to assess the role and alterations of mentioned proteins during VCL-induced apoptosis.   Results: Our results revealed that, the VCL diminished tubular TDI and SPI ratio versus same side control testicles. Moreover, the collateral testicles exhibited up-regulated Bcl-2+, caspase-3+ and PCNA+ cells number per mm2 of tissue compared to sections of the control group. Conclusion: Our data showed that, a- the VCL bilaterally affects the spermatogenesis at least partially by inducing mitochondria -dependent apoptosis and b- the collateral testicle, in contrast to ipsilateral one, exhibits significantly higher anti-apoptotic Bcl-2 and PCNA as well as pro-apototic caspase-3.

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نویسندگان

Mazdak RAZI

Department of Basic Science, Faculty of Veterinary Medicine,Urmia University

ZEYNAB DALIRI

Department of Histology,Ayandeh Research and Diagnostic Company, ACECR

ARAM MINAS

Department of Histology,Ayandeh Research and Diagnostic Company, ACECR

HAMED Rezaiagdam

Department of Histology,Ayandeh Research and Diagnostic Company, ACECR