Adaptive Immunity Play More Important Role Than Innate Immunity in Recurrent Implantation Failure

Introduction: The immune system is required for maternal immune tolerance, protecting the fetus, and regulating the placentation process. In RIF patients, Many factors may contribute to implantation failure as inappropriate immune responses at the time of embryo introduction is one of the reason for unsuccesful implantation. So, evaluation of innate and addaptive immune yatem in RIF patients seems valued. Materials and Methods: Total mRNA were extracted from endometrial tissues of women with repeated implantation failure (n =10) and healthy fertile individuals (n =6) on day 3-5 after ovulation, during luteal phase. The expression profile of 84 genes related to innate and adaptive immunity was investigated using qRT-PCR array. Results: Our data clearly showed that cytokines expressions like IL6, IL-2, IFN gamma, IL17, IL23 and IL13 are higher in RIF group than normal. The innate immunity pathways as, pattern recognition receptors and their pathways like TICAM1, TICAM2, IRAK1, TRAF, MYD88 expression are lower or not significant in RIF than normal group, also the expression of NFKBIA (IκBα, MAD3) as an inhibitor of NFKB1 are higher in RIF group. The adaptive immunity markers like T cell activation,Thelper1 and Thelper17 like CD86, ICAM1, CXCR3, TBX21 and FASLG (TNFSF6) expressions are higher in RIF than normal.Conclusion: our data showed more proinflammatory environment in RIF patients than anti-inflammatory. In addition to, apparently, the source of inflammatory cytokines production are Tcells not PRR signaling pathways. So, it seems adaptive immunity is more contributing factor than innate immunity in pathophysiology of RIF patient

کلیدواژه ها:

Recurrent Implantation Failure ، innate immunity ، Addaptive immunity ، Thelper