In vivo evaluation of response to oxidative stress induced by Hydroxyurea in β-thalassemia major patients

Introduction: Use of HU among beta-thalassemia patients show different clinical outcomes and about 25% of patients do not response to this agent; the objective of this study was to evaluate the possible role of the expression of anti-oxidative genes in response to HU.Material and methods: For miRNAs and mRNAs, RT-PCR was done by using specific primer for each miRNA and random hexamers for mRNAs.Results: In responders, we observed a higher expression of Bcl-xl, Foxo3, ARG1, and miR-451 in comparison to non-Responders. for miR-144, We did not find any difference between the two groups.Conclusion: As Hu can induce production of HbF, activation of genes involved in the regulation of oxidative stress in responders may have a critical role in the survival of erythroid progenitors and maturation. So it can cause to increase Hb levels in responders in comparison to non-responders

کلیدواژه ها:

Hydroxyurea ، Oxidative stress ، miR144/451 ، Foxo3 ، Bcl-xl ، ARG1