Study of interaction between ternary Cu(II) complexof Schiff base and bovine serum albumin by spectroscopic methods

The molecular mechanism of a ternary Cu(II) complexes of schiff-base tryptophan amino acid derivatives and bipyridine hetrocycle(T) binding to bovine serum albumin (BSA) under physiological conditions in vitro under optimal physiological condition (pH=7.4) was investigated by fluorescence quenching and Fourier transform infrared .the fluorescence emission of BSA was quenched by this Schiff base complex that has been analyzed for estimation of binding parameters.Fluorescence quenching data showed that the quenching mechanism of BSA treated by the complex was static quenching, which was highly accord with the non-radioactive energy transfer theory Fourier transform infrared spectroscopic data demonstrated that BSA interacts with Schiff base molecule mainly via both the hydrophobic and hydrophilic interactions with a minor change in the secondary structure of BSA.

کلیدواژه ها:

Schiff base ، BSA ، Flourescence ، FTIR ، interaction