Trans-chalcone and thymol have effect on atheromp Plaque formation in cholesterol-fed NMRI male mice

IntroductionTrans-chalcone and thymol are made up by aromatic ketone and monoterpen flavonoids respectively which have possessed antioxidant and anti-inflammatory properties. The aim of this study was the effects of trans-chalcone and thymol on atheroma plaque formation and biochemical parameters in high cholesterol diet (HCD)-fed NMRI male mice.Methods Fifty NMRI male mice were classified randomly into 7 groups (n = 10 per group). Control group received a normal diet, while group 2 (HCD group) received an additional 2% cholesterol for 18 weeks; and sham group received a HCD for 12 weeks and were then shifted to a normal diet and trans-chalcone and thymol vehicle (sunflower oil) for 6 weeks. Four experimental groups, received a HCD for 12 weeks and were then shifted to a normal diet and either trans-chalcone and thymol (with 12 mg/kg or 24 mg/kg dosage for 6 weeks)Results The aortic sections of all recipient mice that fed with HCD were surveyed by using hematoxylin and eosin staining have showed atheroma plaque formation after 12 weeks. At the end of study, while had significantly increased the major factors in the HCD group: body weight, and serum levels of triglycerides, total-cholesterol, leptin, malondialdehyde. The serum levels of high-density lipoprotein cholesterol, adiponectin and superoxide dismutase had considerably decreased.Conclusions The formation of atheroma plaque was improved by trans-chalcone and thymol via increased expression of adiponectin gene, generation of higher levels of antioxidant enzymes and modulating in lipid serum

کلیدواژه ها:

Trans-chalcone ، Thymol ، Atheroma plaque ، NMRI Male Mice