Effect of tilorone on the mouse hepatoma cell line in presence and absence of culture supernatants from lactobacillus reuteri

Introduction Tilorone dihydrochloride is an anticancer agent with the deferent mechanism. Additionally, the species of Lactobacillus have the important role in anticancer effect. Objectives Because of unknown effects of tilorone and culture supernatants from Lactobacillus reuteri on hepatoma, the aim of this study is the evaluation of apoptotic and anticancer effects of tilorone on mouse hepatoma cell line with and without culture supernatants from L. reuteri. Methods For this purpose, after cell line culture, cells were divided into different groups such as negative control, treatment with 4 invariable doses of tilorone, positive control of supernatant (single dose), and combination therapy groups of different doses of tilorone with supernatant (constant doses), for 48 hours. All groups were studied with pathologic tests, biochemical study, and MTT assay and, absolute real-time PCR was done to assess Bax and Bcl-2 genes expression, as molecular studies. Results MTT assay test showed that tilorone could significant changes in the vitality of treated cell line. Real-time PCR analysis manifested that tilorone dihydrochloride induced up-regulation and down-regulation the expression of Bax and Bcl-2, respectively. Simultaneous, antioxidant effect has seen, in a way that prevents necrosis, in biochemical analysis. These results were in a dose-dependent manner and statistically significant compared to the control group. ConclusionBased on these results, it appeared that this agent could be a good candidate for further evaluation as effective chemotherapy acting through induction of apoptosis in hepatoma. The cell death caused via bacterial supernatant was rather a necrosis than apoptosis

کلیدواژه ها:

Anticancer ، Hepa1-6 cell line ، Tilorone dihydrochloride ، Bax ، Bcl-2