Vildagliptin increase differentiation of insulin producing cells from adipose-derived mesenchymal stem cells in nano scaffold hydrogel

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 321

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ITERMED01_201

تاریخ نمایه سازی: 7 مرداد 1398

چکیده مقاله:

IntroductionType 1 diabetes is caused by destruction of beta cells. Vildagliptin (VG), a dipeptidyl peptidase 4 inhibitor, is an anti-diabetic drug which increases beta cell mass. In present work, VG effect on generation insulin-producing cells (IPCs) from adipose-derived mesenchymal stem cells (ASCs) in a nano hydrogel scaffold was investigated.MethodsIn this experimental study, ASCs were isolated and after characterization were exposed to differentiation media without or with VG in a nano hydrogel scaffold or 3D culture. The presence of IPCs was confirmed by morphology analysis and gene expression (Pdx-1, Glut-2 and Insulin). Newport Green staining was used to determine insulin positive cells. Insulin secretion under different concentration of glucose was measured by using radioimmunoassay method.ResultsIn presence of VG at 3D culture the morphology of differentiated cells was similar to the clusters of pancreatic islet cells. Expression of Pdx-1, Glut-2 and Insulin genes in VG-treated cells was significantly higher than the cells exposed to the only induction media in 3D culture. Insulin release from VG-treated ASCs in the nanoscaffold showed a nearly 3.6 fold (P < 0.05) increase when exposed to a high glucose medium in comparison to the VG-untreated ASCs. The percentage of insulin positive cells in the VG-treated cells was approximately 2.9-fold higher than the VG untreated ASCs.ConclusionThe present study has demonstrated that VG elevates the differentiation of ASCs into IPCs in presence nano hydrogel scaffold. Improvement of this protocol may use to cell therapy in diabetic patients

نویسندگان

Samaneh Karimi

Cell & Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Jafar Ai

Tissue Engineering and Applied Cell Sciences, Department-School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran

Layasadat Khorsandi

Cell & Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran

Darioush Bijannejad

Cell & Molecular Research Center, Faculty of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran