In vitro study of antibacterial and anticancer properties of electrospun nanofibrous polycaprolactone-based scaffolds

Recently, the nanostructured materials have attracted a great attention in biomedical applications due to their significant antibacterial properties. Moreover, nanofibrous scaffolds have gained a notable interest in the field of dermatological applications. In this study, a polycaprolactone-based nanofibrous scaffold has been fabricated using electrospinning method. The aim of this study was to evaluate antibacterial and anticancer properties of electrospun nanofibrous structures. In order to attain homogeneous nanofibrous polycaproclactone-based scaffolds suitable for dermal applications, synthesis parameters including applied voltages, distance of nuzzle to the collector and the rotation speed of drum were optimized. The structure and morphology of polymeric nanofibrous scaffolds were studied by Scanning Electron Microscopy (SEM). In addition, antibacterial properties of nanofibrous scaffolds were studied on two gram-negative bacteria of Escherichia coli and Pseudomonas aeruginosa and two gram-positive bacteria of Staphylococcus urea and Streptococcus strains, using disk diffusion method. Moreover, MTT assay was performed on HT1080 cancerous fibroblastic cell line. The results of this study showed that nanofibrous scaffolds reveal a significant antimicrobial effect on all of the studied bacterial strains, but the most significant effect was observed on Pseudomonas aeruginosa. Cell viability of cancerous cells has drastically decreased after 72 hours to 65% in comparison to the control. Furthermore, absolutely no bacteria attachment on the nanofibrous scaffold has been observed. These results show that electrospun nanofibrous PCL-based scaffolds fabricated in this study are potentially promising for dermal tissue engineering applications, specifically in case of skin cancer treatments.

کلیدواژه ها:

Polycaprolactone ، Scaffold ، Electrospinning ، Nanofibers ، Antibacterial ، Anticancer