Inductive role of sustentacular cells (sertoli cells) conditioned medium on bone marrow derived mesenchymal stem cells

Introduction Male germline stem cells are the key cells to spermatogenesis and fertility. Sustentacular cells (cid, Sertoli cells), as the somatic cell, play a vital role in creating the essential microenvironment for the self-renewal and differentiation of the male germ line cells. Mesenchymal stem cells are recognized as self-renewing and multipotent cells able to differentiate into multiple cell types. Objectives The generation of male germ cells from mesenchymal stem cells by using Sustentacular cell condition medium (cid, SCCM) is a therapeutic method to treat male infertility. Methods Bone marrow-derived mesenchymal cells (cid, BMMSCs) were harvested from the bone marrow of 6-8-weeks (cid, NMRI) mice. Sustentacular cells were isolated and made rich using lectin coated plates. SCCM was collected after different time intervals. Then the BMMSCs were cultured with different concentration of SCCM and DMEM at various times. Results Specific markers of Germ line cells were evaluated using RT-PCR and immunocytochemistry. The results showed that BMMSCs cultured with SCCM for 48h exhibited germ line-specific transcripts (cid, Mvh, Iid4, piwil2) (cid, p< 0.05) and markers (cid, Mvh, Scp3). ConclusionOur findings represent that culturing BMMSCs with SCCM may lead to effective differentiation of BMMSCs into germline cells and provide a treatment strategy for male infertility

کلیدواژه ها:

Sustentacular cells conditioned medium ، Bone marrow-derived Mesenchymal stem cells ، Differentiation ، Culture