In vitro study of the laser irradiation on destruction of breast cancer cells

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 374

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شناسه ملی سند علمی:

ITERMED01_255

تاریخ نمایه سازی: 7 مرداد 1398

چکیده مقاله:

Cancer one of the most important disease in the United States and other countries. Common cancer treatments sometimes cause adverse side effects such as loss of hair, nausea, and weakness. These complications arise because these cancer treatments not only destroy the cancer cells but also damage some of the healthy cells of the body. In an effort to address these complications we want to use laser radiation as a targeted cancer treatment, and in particular, for breast cancer treatment. In this regard, tissue engineering approaches are being employed by using electrospun scaffold in order to support the growth of breast cancer cells. We used Polycaprolactone (PCL), which is biocompatible, biodegradable, and a good supporter of cell growth. The specific breast cancer cells have the ability to create a three-dimensional cell cluster due to the spontaneous accumulation of cells in the porosity of the scaffold under some specific conditions. Therefore, we are looking for a higher density of porosity and larger pore size. Fibers showed uniform diameter distribution and final scaffold had optimum characteristics with approximately 40% porosity. The images were taken by AFM and the density and the size of the porosity were determined with the ImageJ. After scaffold preparation, it has cross-linked by glutaraldehyde. Then it has been washed with glycine and PBS, in order to neutralize the residual glutaraldehyde. MTT results have represented approximately 91.13% viability of the scaffolds for cancer cells. In order to create a cluster, cells were cultured on the scaffold in 24 well plate for several days. The healthy cells of this specific cell line were also cultured with the cancer cells simultaneously. However, they could not grow and propagate in a cluster as much as the cancer cells. Then, we have exposed the cluster to photodynamic laser radiation to investigate the effect of laser on the tumor. It can destroy the tumors under 2cm completely. In conclusion, laser therapy is a viable method to destroy the target cells and has a minimum effect on the healthy tissues and cells. It can improve the other method of cancer treatments limitations.

نویسندگان

Ava Dashti

Faculty of Biomedical Engineering, Amirkabir University of Technology (Tehran Polytechnic)۱, Tehran, Iran.

Mahnaz Eskandari

Amirkabir University of Technology, Tehran, Iran

Parviz Parvin

Recombinant Proteins Department, Breast Cancer Research Centre۳, Motamed Cancer Institute, ACECR, Tehran, Iran

Leila Farahmand

Amirkabir University of Technology, Tehran, Iran