Fibrin scaffold as substrate for culture of bone marrow mesenchymal stem cells

IntroductionMesenchymal Stem Cells (MSCs) are known as appropriate cells in regenerative medicine. Applications of MSCs in clinical practice and regenerative medicine need to keep MSCs as pluripotency stem cells.ObjectivesIn this study, the effect of fibrin scaffold on stemness gene expression of MSCs was examined.MethodsBone marrow-derived MSCs were cultured in tissue culture plates (2D) and 3-dimensional (3D) fibrin scaffolds. The effect of fibrin scaffold on proliferation of MSCs was evaluated by MTT assay. Stemness state was evaluated by qRT-PCR for OCT4, SOX2 genes, and flow cytometry for Nanog protein.ResultsCultured MSCs on fibrin scaffold were able to proliferate according to data obtained by MTT assay. Gene expression of OCT4, and SOX2 had a significant increase in cells were cultured in 3D condition in compared to 2D condition (P<0.05). Also, higher expression of Nanog protein in 3D culture was observed (P<0.05).ConclusionThorough evaluation of important pluripotency regulators such as OCT4, SOX2, and Nanog; we demonstrated that fibrin scaffold could maintain MSCs in a stemness state

کلیدواژه ها:

Mesenchymal stem cells ، Stemness ، Fibrin scaffold ، OCT4 ، SOX2 ، Nanog