Nanogelas a carrier for topical application of vancomycin in infected wound healing

We develop a novel method for the encapsulation of vancomycin hydrochloride into nanogel polymer according to swelling/deswelling mechanism, and the encapsulated vancomycin then mixed with gelling agents to prepare nanogel formulations. The encapsulated nanogel was characterized by using UV-Visible spectrometry, Fourier Transform Infrared (FTIR) spectroscopy, Dynamic light scattering (DLS), Field Emission-Scanning Electron Microscope (FESEM) and Transmission Electron Microscope (TEM) to investigate the content of the drug that encapsulated into nanogel as well as study the effect of pH on particle size and zeta potential of the nanogel polymer. The particle size, zeta potential, polydispersity index and encapsulation efficiency of the optimum 0.125 wt% vancomycin HCl loaded 0.3 wt% nanogel were found to be above 270 nm, -42 mV, 0.239, and 83%, respectively. The in vitro drug release of vancomycin loaded nanogel at different pH dissolution media of phosphate buffer saline was smartly behaved as pH responsive material. At pH 7.5, a fast release of the drug happened within 4 hours while at ten hours, a decrease in the drug release upon adjustment of the pH of the same formula to 6.5. On the other hand, slow and sustained drug release has obtained at pH 5.5 for the third formula. The antibacterial activities of optimized nanogel formulations of ten hydrogel formulas containing four types of gelling agents at different concentration (carboxymethylcellulose, carbopol 974P, xanthan gum, HPMC K100M) were tested towards many of gram positive and gram negative wound pathogens such as such as, Staphylococcus aureus and MRSA. The results proved strong antibacterial activities in comparison with free vancomycin. This research could be a blueprint for enhancing the antibacterial activity for other antibiotics via encapsulating the antibacterial agent into the nanogel which in turn contributes in the development of biomedical applications

کلیدواژه ها:

Nanogelas ، Vancomycin ، Wound healing ،