Detection of Efflux Pump Genes (adeA, adeB, adeC and abeM) in Acinetobacter baumannii Isolated from Hospitalize Patients, North-west of Iran
سال انتشار: 1395
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 325
متن کامل این مقاله منتشر نشده است و فقط به صورت چکیده یا چکیده مبسوط در پایگاه موجود می باشد.
توضیح: معمولا کلیه مقالاتی که کمتر از ۵ صفحه باشند در پایگاه سیویلیکا اصل مقاله (فول تکست) محسوب نمی شوند و فقط کاربران عضو بدون کسر اعتبار می توانند فایل آنها را دریافت نمایند.
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IEM-2-4_002
تاریخ نمایه سازی: 11 تیر 1396
چکیده مقاله:
Background: The importance of this research was to determine the prevalence of efflux pump genes among Acinetobacter baumannii isolates from hospitalized patients in Imam Reza hospital in Tabriz, Iran.Materials and Methods: This descriptive study was conducted in the Imam Reza hospital, Tabriz, IR Iran during June 2013 to March 2014. Twenty-six strains were isolated from female patients (42.6%) and thirty-five from male patients (57.4%). Clinical specimens were culturedfor isolation of the microbial agents of A. baumannii. The isolated bacteria were identified using biochemical tests. Disk diffusionsusceptibility test was used to determine the antimicrobial susceptibility, and E-test methods were also used. The prevalence of efflux pump genes was detected by PCR and sequencing methods.Results: The resistance of A. baumannii isolates against tested antibiotics was analyzed as follows: 51 (84%) to trimethoprimsulfamethoxazole, 59 (98%) to ceftazidime, 60 (99%) to ciprofloxacin, 29 (48%) to amikacin, 46 (77%) to gentamicin, 30 (50%) to tobramicin, , 60 (99%) to imipenem,, 60 (99%) to meropenem,, 60 (99%) to ceftriaxon,, 60 (99%) to cefepime,, 60 (99%) to ofloxacin, 6 (11%) to colistin. By using E-test, 45 (73.3%) to imipenem, 57 (93.3%) to ciprofloxacin, 23 (38%) to amikacin were alsoanalyzed. The prevalence of adeA, adeB, adeC, and abeM genes was 54 (88.5%), 61 (100%), 57 (93.9%), and 60 (98.3%),respectively. Conclusion: The result of this study showed high incidence of AdeABC efflux pump in MDR A. baumannii isolates and the growing number of nosocomial infections associated with XDR A. baumannii complex, leading to difficulties in antibiotic therapy
کلیدواژه ها:
نویسندگان
Goli Angoti
Department of Microbiology, Shahid Beheshti University, M.C International Branch, Tehran, IR Iran
Mojgan Bandehpour
Department of Biotechnology, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran
Hossein Goudarzi
Shahid Beheshti University. M.C. International Branch, Tehran, IR Iran
Maryam Hajizadeh
Department of Microbiology, Imam Reza Hospital, Tabriz, IR Iran