Molecular Typing by Polymerase Chain Reaction Sequence Specific Primers (PCR- SSP) of Human Leukocyte Class I and Class II Alleles in a Sample of Iraqi Visceral Leishmaniasis Patients

سال انتشار: 1393
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 319

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شناسه ملی سند علمی:

JR_IJABBR-2-8_008

تاریخ نمایه سازی: 26 اسفند 1394

چکیده مقاله:

Objective: This study aimed to investigate the association between HLA alleles and visceral leishmaniasis (VL) in a sample of Iraqi patients. Methods: A total of 30 patients were studied, in addition to 20 age, gender and ethnicity matched controls. All subjects were genotyped by polymerase chain reaction-sequence specific primers (PCR-SSP) method. Results: For HLA-class I region (A and B loci), only HLA-A*19 allele showed asignificant (P = 0.031) decreased frequency in VL patients as compared with controls (13.3 vs. 45.0%), and such deviation was associated with OR and PF values of 0.19 and 0.37, respectively. At HLA-class II region, HLA-DRB1*03 and HLA-DQB1*02 alleles were significantly (P = 0.020 and 0.013, respectively) increased in VL patients (56.6 vs. 20% and 46.6 vs. 10%, respectively) as compared with controls. The OR of such two positiveassociations was 5.23 and 7.88, respectively, and the EF value was 0.46 and 0.41, respectively. In contrast, HLA-DRB1*02 (13.3 vs. 45.0%) and HLA-DQB1*03 (33.3 vs. 70.0%) were significantly (P = 0.031 and 0.023, respectively) decreased in patients.However, none of these differences remained significant after correcting the P value forthe number of alleles tested at each locus. Conclusions: these preliminary data suggest that HLA alleles may have some role in aetiopathogenesis of VL, and this role can be in favour of susceptibility and/or protection.

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نویسندگان

Nidal Abdul Mohymen

Department of Microbiology, College of Medicine, Al-Nahrain University, Baghdad, Iraq

Abdullah M. Qader

Biotechnology Research Center, Al-Nahrain University, Baghdad, Iraq

Ali H. Adhiah

Tropical-Biological Research Unit, College of Science, University of Baghdad, Baghdad, Iraq