Investigation on the using of linco-spectin solution for in ovo administration in chicken embryo
سال انتشار: 1393
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 476
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شناسه ملی سند علمی:
JR_IJABBR-2-1_012
تاریخ نمایه سازی: 26 اسفند 1394
چکیده مقاله:
Nowadays antibiotics are used on a large scale in veterinary and human medicine to cure or prevent diseases. Some antibiotics injected into hatching eggs to eliminate pathogens and prevention of egg transmission of disease. Adverse effects of drugs have always been a major concern. There is scantlyinformation available about the safety and pathological alterations of lincosamide-aminocyclitol compounds in embryo. The objective of this study was to investigate using of various dosages of Lincospectin solution for in ovo administration in chicken embryo. Fertile chicken eggs were divided into sixequal treatment groups as follows: group 1: uninjected group. Group 2: needle-injected group; the needle was inserted into the yolk sac without any injection. Group 3: phosphate buffered saline-injected group; whose individuals were injected with phosphate buffered saline. Groups 4, 5 and 6 whose individuals were injected with linco-spectin solution at a dosage of 10 mg lincomycin and 20 mg spectinomycin or 15mg lincomycin and 30 mg spectinomycin or 20 mg lincomycin and 40 mg spectinomycin per Kg eggweight, respectively. Embryos were re-incubated post-treatment and allowed to develop until day 18 after which; they were examined for macroscopic and microscopic lesions. Results showed that embryos werenormal in all treatment groups. Microscopically, no lesions were also diagnosed in tissues. Based on macroscopic and microscopic findings, it is concluded that linco-spectin at above-mentioned concentration is not toxic for the chicken embryo. So, linco-spectin egg-injection can be used to eliminate pathogens and prevention of egg transmission of the disease without any adverse effect.
کلیدواژه ها:
نویسندگان
Hadi Tavakkoli
Department of Avian Medicine, School of Veterinary Medicine, Shahid Bahonar University of Kerman,Kerman, Iran
Amin Derakhshanfar
Department of Pathobiology, School of Veterinary Medicine, Shahid Bahonar University of Kerman,Kerman, Iran
Sajedeh Salandari
School of Veterinary Medicine, Shahid Bahonar University of Kerman, Kerman, Iran