Searching for Alpha-Glucosidase Inhibitory Activity in Hexane Extracts by some Plants from Kurdistan Province

Objective: Alpha-glucosidase, as a carbohydratase catalyses the liberation of alphaglucose from the nonreducing end of some carbohydrates from foods in digestive tract. Consequently, retard absorption of glucose by the inhibition of carbohydrate hydrolyzing enzymes is one of the therapeutic approaches to decrease the postprandialhyperglycemia. Recently, a great deal of attention has been paid to natural products likeplants, as a reliable source for bioactive compounds. The aim of this study was to findnew potent inhibitors for Alpha-glucosidase among plant extracts. Methods: Hexanextracts of 60 plants species were screened for their Alpha-glucosidase inhibitory activity at 0.001, 0.01, 0.1 and 1 mg/mL concentration. Acarbose, dissolved in buffer, wasused as a positive control. Every experiment was done in triplicate. Descurania Sophia(L.) Webb & Berth, Fumaria vailantii Loisel, Ferula Haussknechti Wolf exRech,Haplophyllum acutifolium (DC.)G.Don, Isatis cappadociaca Desv., Eremostachys laevigata Bunge, Silene aucheriana Boiss exhibited more than 60% alpha-glucosidase inhibitory activity.. Results: Among them, Descurania Sophia, Fumaria vailantii andFerula haussknechti exhibited significant alpha glucosidase inhibitory activity with IC50 values of 0.9 μg/ml, 24.55 μg/ml and 2.71 μg/ml, respectively. Because of its high inhibitory activity and low IC50, Descurania Sophia extract would be interesting for further studies.

کلیدواژه ها:

Alpha-glucosidase ، Inhibitor ، Plant extract ، Hexane ، Diabetes mellitus ،