OPTIMIZATION OF LIPASE IMMOBILIZATION

Pseudomonas aeruginosa BBRC-10036 was used for lipase production. Theorganism secreted the enzyme extracellulary. In order to purify the enzyme, precipitationwas carried out first, and then this lipase was purified by Ion Exchange Chromatographyleading to 2.3-fold purification and 11.47% recovery. Lipase from P.aeruginosa wasentrapped into Ca-alginate gel beads and effect of independent variables such as alginateconcentration (%w/v), CaCl2 concentration (M) and enzyme load (%v/v) onimmobilization yield and activity of immobilized enzyme were investigated. Mediaoptimization for immobilization of lipase was carried out by Response Surface Methodology. The optimum conditions were: sodium alginate concentration 2.5% (w/v),calcium chloride concentration 2.5 (M) and enzyme load 50% (v/v). Under theseconditions, the highest immobilization yield and the optimum activity of immobilized enzyme obtained were 93.65% and 2.64 unit/g (IME), respectively

کلیدواژه ها:

Pseudomonas aeruginosa ، Lipase ، Production ، Purification ، Immobilization ، Calcium Alginate