Effect of Transforming Growth Factor-β (TGF-β) on proliferation of gastric epithelial cells in culture

Objective: Helicobacter pylori has a well-established role in the development of gastric cancer. In vitro studies reveal increased proliferation of the gastric mucosa in the presenceof H. pylori infection. It has been also shown that production of some cytokines, such as interleukin-1 beta (IL-1b) is increased in H. pylori infection. In addition, IL-1b increasesproliferation of gastric epithelial cell in culture study. In this study, The effect of transforminggrowth factor beta (TGF-b) on gastric epithelial cell proliferation has been examined. Materials and methods: AGS cells were cultured with TGF-b. DNA synthesis wasevaluated by bromo-deoxyuridine (BrdU) test and total viable cell numbers by MTT assay. Results: TGF-b decreased DNA synthesis and cell numbers. This effect was both timeanddose-dependent (p<0.05). Both tests, BrdU test & MTT assay revealed this suppressiveeffect, but it was more evident in BrdU test. Conclusion: TGF-b suppresses proliferation of malignant gastric epithelial cells. It appearsthat modulatin of tyrosine kinase activity is essential to anti-proliferative effect of TGF-b. Decreased gastric epithelial proliferation due to TGF-β may contribute to the decreased risk of gastric cancer and precancerous lesions in H. pylori-infected individuals or slowing the progression of disease in gastric cancer patients.

کلیدواژه ها:

TGF-β ، gastric epithelial cells