Development of Multiplex Reverse Transcription-Polymerase Chain Reaction for Simultaneous Detection of Influenza A, B and Adenoviruses
محل انتشار: فصلنامه آسیب شناسی ایران، دوره: 13، شماره: 1
سال انتشار: 1397
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 440
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شناسه ملی سند علمی:
JR_IJP-13-1_007
تاریخ نمایه سازی: 23 تیر 1398
چکیده مقاله:
Background and objective:Millions of people in developing countries lose their lives due to acute respiratory infections, such as Influenza A & B and Adeno viruses. Given the importance of rapid identification of the virus, in this study the researchers attempted to design a method that enables detection of influenza A, B, and adenoviruses, quickly and simultaneously. The Multiplex RT PCR method was the preferred method for the detection of influenza A, B, and adenoviruses in clinical specimens because it is rapid, sensitive, specific, and more cost-effective than alternative methods Methods:After collecting samples from patients with respiratory disease, virus genome was extracted, then Monoplex PCR was used on positive samples and Multiplex RT-PCR on clinical specimens. Finally, by comparing the bands of these samples, the type of virus in the clinical samples was determined. Results:Performing Multiplex RT-PCR on 50 samples of respiratory tract led to following results; flu A: 12.5%, fluB: 50%, adeno: 27.5%, negative: 7.5%, and 2.5% contamination. Conclusion: Reverse transcription-multiplex Polymerase Chain Reaction (PCR) technique, a rapid diagnostic tool, has potential for high-throughput testing. This method has a significant advantage, which provides simultaneous amplification of numerous viruses in a single reaction. This study concentrates on multiplex molecular technologies and their clinical application for the detection and quantification of respiratory pathogens. The improvement in diagnostic testing for viral respiratory pathogens effects patient management, and leads to more cost-effective delivery of care. It limits unnecessary antibiotic use and improves clinical management by use of suitable treatment.
کلیدواژه ها:
نویسندگان
Mohsen Nakhaie
Dept. of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Hoorieh Soleimanjahi
Dept. of Virology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran
Hamid Reza Mollaie
Dept. of Medical Microbiology, Kerman University of Medical Sciences, Kerman, Iran
Seyed Mohamad Ali Arabzadeh
Dept. of Medical Microbiology, Kerman University of Medical Sciences, Kerman, Iran
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