Low Expression of Occludin in the Melanoma Patient

سال انتشار: 1398
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 365

فایل این مقاله در 7 صفحه با فرمت PDF قابل دریافت می باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

JR_IJP-14-4_001

تاریخ نمایه سازی: 13 آذر 1398

چکیده مقاله:

Background & Objective: Malignant melanoma is the fatal cutaneous neoplasm which is curable by the early diagnosis. The expression of occludin protein which is an integral membrane protein is altered in an epithelial-to-mesenchymal transition. Although, recent studies provide sufficient evidence supporting the functional importance of occludin in cancer, the prognostic significance of occludin expression levels in melanoma remains obscure. The aim of this study was to determine occludin expression level and itscorrelation with clinicopathological features of the patients with melanoma.  Methods: The occludin mRNA level was compared between paraffin-embedded tissues of 40 patients with melanoma and 10 subjects with normal skin. The quality and quantity of the RNA was determined and occludin expression level was measured using Real-time PCR and ∆∆CT computational technique. Results: Theoccludin mRNA level reduced five-fold in the melanoma patients compared to the control group (P=0.000). No significant difference was observed between male and female cases (P=0.533). No significant correlation was observed between occludin mRNA level, mitotic count (P=0.252), and Breslow levels (P=0.171) Conclusion: We can conclude that down-regulation of occludin expression in the patients with melanoma is a hallmark of cancer progression and it might be used as a prognostic factor. No significant correlation was found between occludin gene expression and clinicopathological characteristics including Clark level, Breslow staging, mitotic count, age and gender (P<0.05).

کلیدواژه ها:

نویسندگان

Pouri Salehi

Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran

Farzaneh Tafvizi

Department of Biology, Parand Branch, Islamic Azad University, Parand, Iran

Kambiz Kamyab Hesari

Department of Pathology, Tehran University of Medical Sciences, Tehran, Iran

مراجع و منابع این مقاله:

لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :
  • McCoy ML, Mueller CR, Roskelley CD. The role of the ...
  • Feng S, Huang Y, Chen Z. Does VEGF secreted by ...
  • Thompson JF, Scolyer RA, Kefford RF. Cutaneous melanoma in the ...
  • Curtin JA, Busam K, Pinkel D, Bastian BC. Somatic activation ...
  • Broekaert SM, Roy R, Okamoto I, Van Den Oord J, ...
  • Jayagopal A, Yang JL, Haselton FR, and Chang MS. Tight ...
  • Flaherty KT, Hodi FS, Fisher DE. From genes to drugs: ...
  • Donizy P, Kaczorowski M, Halon A, Leskiewicz M, Kozyra C, ...
  • Bhat AA, Uppada S, Achkar IW, Hashem S, Yadav SK, ...
  • Coradini D, Casarsa C, Oriana S. Epithelial cell polarity and ...
  • Paschoud S, Bongiovanni M, Pache JC, Citi S. Claudin-1 and ...
  • Al-Sadi R, Khatib K, Guo S, Ye D, Youssef M, ...
  • Buschmann MM, Shen L, Rajapakse H, Raleigh DR, Wang Y, ...
  • De Benedetto A, Rafaels NM, McGirt LY, Ivanov AI, Georas ...
  • Du D, Xu F, Yu L, Zhang C, Lu X, ...
  • Furuse M, Moriwaki K. The role of claudin-based tight junctions ...
  • Turksen K, Troy TC. Junctions gone bad: Claudins and loss ...
  • Martin TA, Mansel RE, Jiang WG. Loss of occludin leads ...
  • Kominsky SL, Argani P, Korz D, Evron E, Raman V, ...
  • Sehrawat A, Singh SV. Benzyl isothiocyanate inhibits epithelial-mesenchymal transition in ...
  • Qin Z, Fang D, Fang Y. Low expression of occludin: ...
  • Vollmer RT. A Probabilistic Analysis of Mitotic Counts in Melanoma. ...
  • Mahdavi T, Tafvizi F, Keyri Manjili HR. Increased MicroRNA-362 level ...
  • Ahmadi M, Tafvizi F, Moslemi E. Comparison of c-Myc expression ...
  • Garbe C, Leiter U. Melanoma epidemiology and trends. Clin Dermatol ...
  • Wood C. Travel health: sun protection and skin cancer prevention ...
  • Brusselbach S, Mohle-Steinlein U, Wang ZQ, Schreiber M, Lucibello FC, ...
  • Rachow SM, Zorn-Kruppa U, Ohnemus N, Kirschner S, Vidal-y-Sy P, ...
  • Guillemot L, Citi S. Cingulin regulates claudin-2 expression and cell ...
  • Gupta I, Ryan A. Claudins: unlocking the code to tight ...
  • Brandner JM, Zorn-Kruppa M, Yoshida T, Moll I, Beck LA, ...
  • Fazakas C, Wilhelm I, Nagyoszi P, Farkas AE, Haskó J, ...
  • Fasano A. Zonulin and its regulation of intestinal barrier function: ...
  • Martin TA, Mason MD and Jiang WG. Tight junctions in ...
  • Tsukita S, Yamazaki Y, Katsuno T and Tamura A. Tight ...
  • Martin TA, Jordan N, Davies EL and Jiang WG. Metastasis ...
  • Xiong L, Wen Y, Miao X and Yang Z. Expressions ...
  • Chen H, Zhang S, Zhen K and Wang XF. [Relationship ...
  • Ohtani S, Terashima M, Satoh J, Soeta N, Saze Z, ...
  • Nemeth Z, Szasz AM, Somoracz A, Tatrai P, Nemeth J, ...
  • Najafi M, Alizadeh SA, Azad M, Naserpour Farivar T, Rajaei ...
  • Beeman N, Webb PG, Baumgartner HK. Occludin is required for ...
  • نمایش کامل مراجع