Detection of Methicillin Resistance in Staphylococcus aureus by Disk Diffusion and PCR Methods
محل انتشار: فصلنامه آسیب شناسی ایران، دوره: 3، شماره: 1
سال انتشار: 1387
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 681
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شناسه ملی سند علمی:
JR_IJP-3-1_011
تاریخ نمایه سازی: 5 آبان 1393
چکیده مقاله:
Background and Objective: Methicillin resistance in Staphylococcus aureus is an increasingly important clinical problem. A chromosomal gene, mecA, mediates resistance to penicillinase-resistantpenicillins such as methicillin and oxacillin in Staphylococcus aureus. We evaluated the validityof disk diffusion test by using oxacillin, methicillin and cefoxitin disks with consideration of the presence of mecA gene as the reference method for detection of methicillin resistant Staphylococcusaureus (MRSA). Materials and Methods: The susceptibility testing of 222 S. aureus clinical isolates to oxacillin(1 μg), cefoxitin (30 μg) and methicillin (5 μg) was carried out by the disk diffusion method according to the Clinical Laboratory Standards Institute guidelines. Detection of mecA gene was performedusing PCR method. Results: An amplified mecA gene of 310 bp was detected in 55% of examined strains by PCR,thus 55% strains were considered MRSA. Sensitivity of oxacillin, methicillin and cefoxitin disks were determined 100%, 99.1% and 98.3% respectively.Conclusion: All MRSA strains in PCR had shown resistance to penicillinase-resistant penicillins by oxacillin disk, but two and one strains were sensitive by cefoxitin and methicillin disk respectively. Thus, oxacillin was the most appropriate disk for detecting MRSA. The prevalence of MRSA in thisstudy is comparable to that found in United States, Canada, Europe and Iran, but the percentage of MRSA isolates is almost twice of percentage reported from Japan.
کلیدواژه ها:
نویسندگان
Mehri Habibi
Dept. of Microbiology, School of Medicine, Shahed University, Tehran, Iran
Horieh Saderi
Dept. of Microbiology, School of Medicine, Shahed University, Tehran, Iran
Parviz Owlia
Dept. of Microbiology, School of Medicine, Shahed University, Tehran, Iran
Mohammadreza Asadi Karam
Dept. of Biotechnology, Pasteur Institute of Iran, Tehran, Iran