The Effect of Murine Leukemia Inhibitory Factor on In Vitro Differentiation of Mouse Embryonic Stem Cells
محل انتشار: مجله طب تولید مثل ایران، دوره: 1، شماره: 1
سال انتشار: 1382
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 301
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شناسه ملی سند علمی:
JR_IJRM-1-1_003
تاریخ نمایه سازی: 16 شهریور 1395
چکیده مقاله:
Background: Embryonic stem cells (ESc) are pluripotent cells which have been used as a model to study the mechanism that control the embryogenesis and early mammalian development in vitro.The aim of this study was to isolate and produce embryonic stem cells from late blastocyst stage embryos in mice. Materials and Methods: Blastocyst stage embryos from pregnant NMRI mice were obtained and cultured for 24 h in DMEM medium. 4-6 days after hatching, the inner cell masses (ICM) formedcolonies which were then collected mechanically and trypsinized. Several subcultures were preparedin the medium supplemented with 0.1 mM 2 Mercaptoethanol, 1000 U/ml Leukemia Inhibitory Factor (LIF) and 10% Fetal Bovine Serum (FBS). The (ESc) were recognized by alkaline phosphates histochemistry using azo-coupling method.Results: The results demonstrated that a highly pluripotent stem cell line was derived from theblastocyst stage embryos of NMRI mice; however, the rate of colonies was as low as 10%.Conclusion: The LIF is effective to culture and maintain the isolated ICM colonies in undifferentiated condition in the absence of feeder layer
کلیدواژه ها:
نویسندگان
Ali A. Movassagh-Pour
Department of Hematology,
Mojdeh Salehnia
۲Department of Embryology
Ali A. Pourfatollah
Department of Hematology, Department of Immunology, School of Medical Sciences,Tarbiat Modarres University, Tehran, Iran
Sayed M. Moazzeni
Department of Immunology, School of Medical Sciences,Tarbiat Modarres University, Tehran, Iran