Detection of Infectious Laryngotrachities Virus PCR and Nested PCR
محل انتشار: مجله بین المللی مطالعات علمی، دوره: 1، شماره: 2
سال انتشار: 1394
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 401
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شناسه ملی سند علمی:
JR_IJSS-1-2_007
تاریخ نمایه سازی: 9 خرداد 1396
چکیده مقاله:
n this study a polymerase chain reaction (PCR) was optimized for rapid and sensitive detection of infectious Laryngotrachietis (ILTV). A 443bp DNA fragment of ILTV was amplified. After DNA extraction, PCR was carried out using specific primers and then specificity of the results was confirmed by sequencing of PCR products. Sensitivity of the test was found to be 3 210 EID50 by this method. After using Nested-PCR a 201bp fragment amplified which increased the sensitivity of the test 210 1EID50... using this technique has many advantages compared with other methods that are currently used. Therefore, it can be used as a reliable diagnostic test for rapid detection of ILTV infections.
کلیدواژه ها:
نویسندگان
A Alamian
Poultery Research and Vaccines Production Department, Razi &Serum Research Institute,P.O.Box;11365-1558,Tehran,Iran
A Ghoreshi
National Research Center for Genetic Engineering and Biotechnology P.O.Box; 14155-6343,Tehran,Iran