Prevalence, molecular characterization and serology of Shiga toxinproducing Escherichia coliisolated from buffaloes in WestAzerbaijan, Iran
محل انتشار: فصلنامه طب دامی ایران، دوره: 5، شماره: 2
سال انتشار: 1390
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 372
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شناسه ملی سند علمی:
JR_IJVM-5-2_009
تاریخ نمایه سازی: 26 اردیبهشت 1394
چکیده مقاله:
This present study is the first to report the presence of Shiga toxinproducing (STEC) in buffaloes in Iran.Atotal of 360 fecal samples were collected from buffaloes from different regions in the west Azerbaijan province of Iran and cultured for the isolation of using routine biochemical tests. From the fecal samples, 340 were isolated and, of these, 26 STEC isolates were identified. The STEC isolates were further analyzed for the presence of specific virulence genes. Among the STEC isolates, 11 (42.3%) isolates were positive for the gene, nine (34.6%) were positive for the gene and six (23%) were positive for bothof these genes. Six (23%) STEC isolates harbored the gene and two (7.6%) isolates were positive for the gene. Based on serotyping, only one (3.8%) isolate was of the O157 serotype, while the other 25 (96.1%) belonged to non-O157 serotypes. The results of the present study provide the first evidence that buffaloes could be a reservoir for STEC in Iran, especially those belonging to non-0157 serotypes
کلیدواژه ها:
نویسندگان
N Yaghobzadeh
Department of Basic Veterinary Science, Faculty of Veterinary Medicine, Razi University, Kermanshah, Iran
A. Ownagh
Department ofMicrobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
K Mardani
Department of Food Hygiene and Quality Control,Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
M Khalili
of Pathobiology, Faculty of Veterinary Medicine, ShahidBahonar University of Kerman, Kerman, Iran.