An evaluation of some oxidative and enzymatic biomarkers in different stages of naturally occurring copper poisoning in sheep

سال انتشار: 1394
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 376

فایل این مقاله در 6 صفحه با فرمت PDF قابل دریافت می باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

JR_IJVM-9-1_002

تاریخ نمایه سازی: 19 اردیبهشت 1395

چکیده مقاله:

BACKGROUND: The early stage of Copper poisoning is difficultto be clinically diagnosed in sheep and has not been documentedclearly yet. OBJECTIVES: To assess biomarkers in predictingearly Copper poisoning in sheep, blood samples werecollected from several groups of animals from a naturally Copperpoisoning occurrence in an industrial region. METHODS:Animals were divided into four experimental groups; GroupA: far from polluted region (Control group), Group B: insidepolluted region, apparently healthy and without any clinicallyjaundice manifestation, Group C: slightly with jaundice signsand hemolytic crisis phase, and Group D: with clear jaundicesigns. After collecting blood samples from each group, the serumwas analyzed for evaluation of liver enzymes and oxidativestress parameters in different stages of Copper poisoning.In each blood sample, CPK, GGT, AST, ALT, total thiol (T-SH)group, and total proteins were determined. The Copper concentrationin the serum, liver, and kidney of the dead animalsin group D were also determined. RESULTS: There were significantdifferences in the blood parameters in group C illustratedby elevated level of serum AST, CPK, and GGT activities andtotal thiol (as biomarker of oxidative stress) when compared tocontrol groups. In group D, these enzymes, in addition to T-SH,and the total protein were significantly (p? 0.05) different fromthose of the control and the other groups. Measurement of Copperin serum, liver, and kidney of group D (at the end stage ofhemolytic phase) confirmed Copper poisoning in these groups.CONCLUSIONS: Based on the findings of the present study, themeasurement of the liver enzyme activities and total thiol justclosed to critical hemolytic phase could be reliable biomarkersfor predicting Copper poisoning in sheep.

کلیدواژه ها:

نویسندگان

J Salar-amoli

Toxicology and Poisoning Research Center, Department of Toxicology, Faculty of Veterinary Medicine,University of Tehran, Tehran, Iran

S Yazdani

Department of Internal Medicine, University of Groningen, Groningen, the Netherlands

T Aliesfahani

Toxicology and Poisoning Research Center, Department of Toxicology, Faculty of Veterinary Medicine,University of Tehran, Tehran, Iran

N Ranjbar

Graduated from the Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran