Development of PCR-Based Method for Rapid Detection of Abrin Gene
سال انتشار: 1395
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 414
متن کامل این مقاله منتشر نشده است و فقط به صورت چکیده یا چکیده مبسوط در پایگاه موجود می باشد.
توضیح: معمولا کلیه مقالاتی که کمتر از ۵ صفحه باشند در پایگاه سیویلیکا اصل مقاله (فول تکست) محسوب نمی شوند و فقط کاربران عضو بدون کسر اعتبار می توانند فایل آنها را دریافت نمایند.
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_JABR-3-3_007
تاریخ نمایه سازی: 16 شهریور 1399
چکیده مقاله:
Abrin, known as a ribosome inactivating protein (RIP), is a high cytotoxic plant protein. The high lethality, low cost, and easy access to this plant and its seeds have led to this toxin to be used in crimes and terrorist acts. Since, obtaining purified toxins requires advanced laboratory equipment and complex procedures, it seems that the perpetrators of such crimes use crude extracts. As a result, it was hypothesized that remaining the specific toxin genes in these extracts can provide the advantage of using PCR assay to identify abrin gene which refers to the existence of its toxin. We used a new rapid molecular method for the detection of the abrin gene by PCR. In this regard, specific primers were designed and the required DNA was extracted from Rosary pie samples using cetyltrimethylammonium bromide-polyvinylpyrrolidone (CTAB-PVP) method and PCR protocol was performed using specific primers. Then, assay’s sensitivity was analyzed using serial dilution method. The results of this study revealed that designed and selected primers sequence for toxin’s gene function as specific. The desired product size was obtained and sequencing of PCR products showed up to 90% similarity with known sequence for each molecule. According to these results, the developed rapid molecular method for detection of abrin toxin gene can be considered as a sensitive and low-cost detection method for this toxin gene in cases of suspection to bioterrorism event.
کلیدواژه ها:
نویسندگان
Morteza Hamedi Behnaq
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Ali Karami
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Ali Choopani
Applied Biotechnology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran|Department of Biology, Payam Noor University, Isfahan, Iran