Molecular Epidemiology and Drug Resistance Study of Entamoeba histolytica in Clinical Isolates from Tehran, Iran
سال انتشار: 1395
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 352
فایل این مقاله در 5 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_JABR-3-4_007
تاریخ نمایه سازی: 16 شهریور 1399
چکیده مقاله:
Amoebiasis is a disease caused by Entamoeba histolytica, a protozoan parasite. Metronidazole is known as the main drug used for patients suffering from Amoebiasis. Despite the lack of drug resistance in clinical samples, there are scattered reports that are based on the failure of treatment which show the increase of clinical drug resistance against metronidazole. Therefore, the aim of this study was to identify E. histolytica by culture and polymerase chain reaction methods, compare them, and assess drug resistance among clinical samples to E. histolytica. A total of 1990 samples were collected from patients with dysentery. Positive microscopic samples after staining by lugol’s iodine were cultured on biphasic culture medium (HSre+s). The drug sensitivity of clinical isolates and standard reference strain of E. histolytica (HM1:IMSS) was evaluated after exposure to various concentrations of metronidazole on the basis of mobility and tonality using 0.01% Eosin. A PCR method was applied to confirm the cultural results. Forty six out of 19990 samples and 41 out of 46 samples were positive for E. histolytica by microscopic and cultural methods, respectively. However, only 15 out of 46 samples were positive by PCR amplification using specific primers of E. histolytica genome. According to the results of Entamoeba growing in the cultures with difference metronidazole concentrations, no resistance was observed at the concentrations higher than 2 mg/ml. The present results indicate the high specificity of the molecular techniques against culture in specific mediums. It also suggests Entamoeba isolates in Iran does not seem resistant to the metronidazole antibiotic.
کلیدواژه ها:
نویسندگان
Arash Hemmati
Department of Microbiology, Rasht Branch, Islamic Azad University, Rasht, Iran
Ali Choopani
Applied Microbiology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran|Department of Biology, Payamnoor University, Tehran, Iran
Fatemeh Pourali
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
Seyed Mohammad Javad Hosseini
Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, Iran
مراجع و منابع این مقاله:
لیست زیر مراجع و منابع استفاده شده در این مقاله را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود مقاله لینک شده اند :