بهینه سازی استخراج ترکیبات ضداکسیدانی جلبک قهوه ای Sargassum angustifoliumبا رفلاکس حرارتی در روش تاگوچی

سال انتشار: 1395
نوع سند: مقاله ژورنالی
زبان: فارسی
مشاهده: 277

فایل این مقاله در 13 صفحه با فرمت PDF قابل دریافت می باشد

استخراج به نرم افزارهای پژوهشی:

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شناسه ملی سند علمی:

JR_JAPU-5-3_001

تاریخ نمایه سازی: 12 آبان 1398

چکیده مقاله:

در این مطالعه از روش رفلاکس حرارتی برای استخراج عصاره از جلبک قهوه ای سارگاسوم استفاده شد. استخراج با 3 حلال (اتانول، آب/اتانول و آب) و در 3 زمان (90، 180 و 270 دقیقه) انجام شد. آزمایش ها با استفاده از طرح تاگوچی در سه سطح انجام شد. میزان فنول کل و خنثی کنندگی رادیکال آزاد سنجیده شد. با توجه به نتایج، بهترین شرایط برای استخراج ترکیبات ضداکسیدانی از سارگاسوم استخراج آبی و زمان 270 دقیقه بود. میزان فنول کل و IC50 در شرایط بهینه 392 میلی گرم اسید گالیک بر گرم ماده خشک (GAE) و 008/0 میلی گرم عصاره بود. نتایج نشان داد که این جلبک برای استخراج ترکیبات ضداکسیدانی مناسب می باشد.

نویسندگان

آریا باباخانی لشکان

دانشگاه گیلان

زهرا نحوی

تربیت مدرس

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