A Novel Signal Peptide Derived from Bacillus Licheniformis α-Amylase Efficiently Targets Recombinant Human Activin A to the Periplasm ofEscherichia coli
محل انتشار: مجله سلول و تحقیقات مولکولی، دوره: 10، شماره: 1
سال انتشار: 1397
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 364
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شناسه ملی سند علمی:
JR_JCMR-10-1_007
تاریخ نمایه سازی: 29 اسفند 1398
چکیده مقاله:
Human activin A is a member of the transforming growth factor-β superfamily consists of two similar beta subunits.Activin A is expressed by different cells and displays numerous biological activities such as control of neuronal cellproliferation and differentiation, promotion of neuronal survival in the body. Therefore, recombinant production ofactivin A is beneficial because it can be used to treat many neurodegenerative diseases such as Alzheimer s andParkinson diseases. In this study E. coli as a cheap and fast-growing host was selected to produce recombinant humanactivin A. As cytoplasmic expression of human activin A with complex structure and disulfide bonds producesinclusion bodies, so periplasmic expression of it can be beneficial. Therefore, we used modified Iranian B.licheniformis α-amylase signal peptide as a new signal peptide in order to translocate the recombinant activin Athrough the inner membrane. In this study human pro-activin A cDNA and signal sequence were cloned in pET21bvector and resulting vector transformed into the two strains of E. coli BL21. SDS-PAGE and western blot techniqueswere used to confirm recombinant activin A expression. Finally, our results indicated that the signal peptide used inthis study was effective for secretion of activin A into the periplasmic space of E. coli.
کلیدواژه ها:
نویسندگان
Zahra Hajihassan
Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran
Seyed Kazem Hosseini
Department of Life Science Engineering, Faculty of New Sciences and Technologies, University of Tehran, Tehran, Iran
Alireza Zomorodipour
Molecular Biotechnology Department, National institute of Genetic Engineering and Biotechnology, Tehran, Iran