Analysis of Suitable Signal Peptides for Designing a Secretory Thermostable Cyanide Degrading Nitrilase: An in Silico Approach

Secretory production of recombinant proteins has many benefits such as solubility and ease of purification. The aim of this study was to find suitable signal peptides for secretory production of nitriles in Bacillus subtilis. The signal peptides were chosen from Signalpeptide web server. SignalP server was used to define probability of suitable signal peptides and their secretion pathways. Physico-chemical properties and solubility were predicted by ProtParam and Protein-sol, respectively. Bioinformatics analysis identified SUBF_BACSU, GUB_BACSU, SACB_BACAM and AMY_BACAM that linked to nitriles are appropriate signal peptides. Their fusion proteins could be stable, soluble and non-antigenic proteins that might have suitable secondary and tertiary structures. The recommended signal peptides by this study are appropriate for rational designing of secretory soluble nitriles. Thus, the results of present work can be useful for future experimental production of secretory and soluble nitriles

کلیدواژه ها:

In silico ، Nitrilase ، Cyanide ، Signal Peptide.