A new protocol for direct regeneration of stevia plant (Stevia rebaudiana Bertoni) by tissue culture techniques
سال انتشار: 1397
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 346
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شناسه ملی سند علمی:
JR_JHPR-1-2_003
تاریخ نمایه سازی: 22 مهر 1398
چکیده مقاله:
Purpose: Due to pharmaceutical value of Stevia plant (Stevia rebudiana Bertoni.), this study was done to introduce a new protocol for rapid mass propagation of itthrough tissue culture. Research Method: In MS medium shoot proliferation of stevia by six concentrations of BA (0, 0.1, 0.2, 0.3, 0.4, 0.5 mg·l-1) and root induction by four concentrations of IBA (0, 0.025, 0.05, 0.1 mg·l-1) was investigated.Rooting of cuttings was done both in vitro and ex vivo conditions. Findings: According to the results, the most number of stems obtained in MS medium containing 0.3 mg l-1 BA. The highest length of stems obtained in MS medium without BA and the most number of leaves observed in MS medium supplemented with 0.4 mg·l-1 BA. In in vitro situation, the most number and length of roots obtained in MS medium without IBA. The most number of rooted cuttings was obtained in IBA solution after 72 hours and about 70% of rooted cuttings were healthy. Research limitations: It had no limitation to report. Originality/value: In conclusion, it seems that the potential of producing root and shoots in stevia plant is extremely high, so its proliferation is possible using low concentrations of plant growth regulators in in vitro culture.
کلیدواژه ها:
نویسندگان
Soghra Rezaie
Department of Horticultural Science, Faculty of Agriculture and Natural Resources, Ardakan University, Ardakan, Iran
Maryam Dehestani-Ardakani
Department of Horticultural Science, Faculty of Agriculture and Natural Resources, Ardakan University, Ardakan, Iran
Kazem Kamali
Department of Soil Science, Faculty of Natural Resources, Yazd University, Yazd, Iran
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