Fingerprinting and genetic diversity evaluation of rice cultivars usingInter Simple Sequence Repeat marker

سال انتشار: 1394
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 598

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شناسه ملی سند علمی:

JR_JPMB-3-1_007

تاریخ نمایه سازی: 16 فروردین 1395

چکیده مقاله:

Rice as one of the most important agricultural crops has a putative potential for ensuring food securityand addressing poverty in the world. In the present study, in order to provide basic information toimprove rice through breeding programs, Inter Simple Sequence Repeat marker (ISSR) was used ForDNA fingerprinting and finding genetic relationships among 32 different cultivars. In this study, 12 out of17 used primers amplified 184 distinct and reproducible fragments with high value of polymorphism(88%). also, for fingerprinting the cultivars 29 loci were used that generated high polymorphic bandsamong the cultivars. Results indicated that similarity index varied between 39% and 88.4 %, furthermore,PIC value with an average of 23% ranged between 0.1 (for primer #3) to 0.34 (for primer #2). Clusteringbased on Jaccard coefficient similarity index and UPGMA algorithm divided the cultivars into 6 mainsub-clusters in cut-off point of 64% similarity index. The two Italian rice cultivars ‘Ribe’ and ‘Roma’were the closest cultivars in addition, ‘Vialone nano’ and ‘Anbarbu’ showed the highest dissimilarity. Intotal, high genetic divergence was observed among the cultivars also, poly (GA)-containing 3-anchoredprimers amplified the highest number of bands. According to similarity and cluster analysis, it could beinferred that crosses involving Anbarbu cultivar are the most promising ones to improve rice throughbreeding programs. In fact, results of this study would be promising as a genetic marker for theidentification of rice cultivars and an important source of knowledge for subsequent rice researches.

نویسندگان

Behzad Shahin Kaleybar

Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari AgriculturalSciences and Natural Resources University, Sari, Iran

Sara Kabirnattaj

Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari AgriculturalSciences and Natural Resources University, Sari, Iran

Ghorban Ali Nematzadeh

Genetics and Agricultural Biotechnology Institute of Tabarestan (GABIT), Sari AgriculturalSciences and Natural Resources University, Sari, Iran

Kamal Kazemitabar

Department of Agronomy and Plant Breeding, Sari Agricultural Sciences and NaturalResources University, P.O. Box 578, Sari, Iran