In vitro Plant Regeneration of Helianthus Annuus (Hyb. Azargol) From Alginate-Encapsulated Shoot Tips for Short Term Storage, Germplasm Exchange and Distribution
سال انتشار: 1395
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 390
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شناسه ملی سند علمی:
JR_JPMB-4-2_001
تاریخ نمایه سازی: 15 اردیبهشت 1397
چکیده مقاله:
The present study demonstrates the potential of nutrient-alginate encapsulation of shoot tips of sunflower, Helianthus annuus (hyb. Azargol) for synthetic seed technology, which could be useful in germplasm distribution and exchange. Shoot tips from in vitro shoot cultures derived from mature seed explants were encapsulated in 3% sodiumalginate and 100 mM CaCl2. 2H2O are supplemented with three different matrices (include distilled water, liquid MS medium and plant growth regulators) and they are stored for several periods (15, 30, 45 and 60 days) at 4°C. After each storage period for regeneration and regrowth evaluation, encapsulated and non-encapsulated shoot tips were cultured on hormone-free MS medium. The regrowth ability of encapsulated shoot tips affected by the storage duration and the presence or absence of MS nutrients in calcium alginate beads. Percentage response for the conversion of encapsulated and non-encapsulated shoot tips decreased gradually after storage at 4°C by increasing storage durations. Indeed, encapsulated vegetative propagules showed a higher resistance to storage at 4°C than non-encapsulated. Addition of MS nutrients in calcium alginate beads significantly improved encapsulated explants regrowth after storage periods
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نویسندگان
Soheila Moradi
Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
Mohammadreza Azimi
Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
Fariborz Habibi
Department of Horticulture, Faculty of Agriculture, University of Zanjan, Zanjan, Iran
Saied saeed Pourdad
Dryland Agricultural Research Institute, Sararood, Kermanshah, Iran