Comparison of Enzyme Prodution Rate of Extracted Native Bacteria from Saline and Alkaline Soil

12 stain of alkalophilic bacteria extracted from saline and alkaline soils of Azerbaijan province in milk agar environment due to evaluate alkaline protease enzyme production feasibility. Amongst isolated bacteria, Bacillus genus members revealed higher product ability. Studying temperature, incubation time, carbon source, nitrogen and pH demonstrated that the best production condition is on 40 oC for 20 hours using 1.5% glucose (weight to volume), 1% Ammonium sulfate (weight to volume) and pH=9.5.

کلیدواژه ها:

Couroupita guianAlkaline Protease ، Bacillus Species ، Alkalophileensis ، genetic diversity ، Primers ، RAPD – PCR