Antioxidant Activity and High Performance Liquid Chromatography Analyzation of Methanolic and Aqueous Extract of Stachys Inflata

سال انتشار: 1393
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 490

فایل این مقاله در 8 صفحه با فرمت PDF قابل دریافت می باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

JR_OCJ-2-1_001

تاریخ نمایه سازی: 19 شهریور 1396

چکیده مقاله:

The objective of this paper was a screening of polyphnol and flavonoid by spectrophotometer. The type of some polyphenols in Stachys inflata was determined by high performance liquid chromatography (HPLC). The methanol extract of plant material was used for HPLC analysis. The HPLC system was coupled with colorimetric detection of antioxidant activity. Additionally, the antioxidant activities were determined by ferric reducing antioxidant power (FRAP) and 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical scavenging activity. The flavonoid and total phenolic contents of methanolic extract was higher than aqueous extract of Stachys inflata. A strong positive correlation of R2 = 0.90 between total phenolic content and antioxidant activity was observed in this investigation. This study indicated that Stachys inflata exhibite high antioxidant activity, flavonoid and phenolic content and can be used potentially as a readily accessible source of natural antioxidant. Antioxidant activities of polyphenols have been suggested to exert beneficial pharmacological effects on some diseases

نویسندگان

Sayyed Mehdy Hashemi Sohi

Young researcher club, Saveh branch, Islamic Azad University, Saveh, Iran

Anoosh eghdami

Biology Department of Saveh branch, Islamic Azad University, Saveh, Iran

Fathemeh sadeghi

Biology Department of Saveh branch, Islamic Azad University, Saveh, Iran