Cutibacterium Acnes is Isolated from Air Swabs: Time to Doubt the Value of Traditional Cultures in Shoulder Surgery
محل انتشار: مجله استخوان و جراحی عمومی، دوره: 8، شماره: 4
سال انتشار: 1399
نوع سند: مقاله ژورنالی
زبان: انگلیسی
مشاهده: 313
فایل این مقاله در 5 صفحه با فرمت PDF قابل دریافت می باشد
- صدور گواهی نمایه سازی
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_TABO-8-4_005
تاریخ نمایه سازی: 22 تیر 1399
چکیده مقاله:
Background: Given high rates of positive Cutibacterium acnes (C. acnes) cultures in cases of both primary and revisionshoulder surgery, the ramifications of positive C. acnes cultures remain uncertain. Next generation sequencing (NGS)is a molecular tool that sequences the whole bacterial genome and is capable of identifying pathogens and the relativepercent abundance in which they appear within a sample. The purpose of this study was to report the false positiveculture rate in negative control specimens and to determine whether NGS has potential value in reducing the rate offalse positive results.Methods: Between April 2017 and May 2017 swabs were taken during primary shoulder arthroplasty. After surgicaltime out, using sterile gloves, a sterile swab was opened and exposed to the air for 5 seconds, returned to its contained,and sealed. One swab was sent to our institution’s microbiology laboratory for aerobic and anaerobic culture and heldfor 13 days. The other sample was sent for NGS (MicroGen Dx, Lubbock, TX), where samples were amplified forpyrosequencing using a forward and reverse fusion primer and matched against a DNA library for species identification.Results: For 40 consecutive cases, swabs were sent for culture and NGS. C. acnes was identified by culture in 6/40(15%) swabs and coagulase negative staphylococcus (CNS) was identified in 3/40 (7.5%). Both cases with positiveNGS sequencing reported polymicrobial results with one sample (2.5%), including a relative abundance of 3% C.acnes. At 90 days after surgery, there were no cases of clinical infection in any of the 40 cases.Conclusion: We demonstrate that the two most commonly cultured organisms (C. acnes and CNS) during revisionshoulder arthroplasty are also the two most commonly cultured organisms from negative control specimens.Contamination can come from air in the operating room or laboratory contamination.Level of evidence: III
کلیدواژه ها:
نویسندگان
Surena Namdari
Rothman Institute, Thomas Jefferson University, Department of Orthopaedic Surgery, Philadelphia, PA, USA
Thema Nicholson
Rothman Institute, Thomas Jefferson University, Department of Orthopaedic Surgery, Philadelphia, PA, USA
Javad Parvizi
Rothman Institute, Thomas Jefferson University, Department of Orthopaedic Surgery, Philadelphia, PA, USA