ALTERATIONS OF CREB GENE EXPRESSION DURING BUPRENORPHINE MODULATED METHAMPHETAMINE ADDICTION IN RAT

سال انتشار: 1398
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 419

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شناسه ملی سند علمی:

KAMED13_042

تاریخ نمایه سازی: 10 دی 1398

چکیده مقاله:

Background and Aim : Methamphetamine is a family of amphetamines (C6H13N), a stimulant and addictive substance that greatly stimulates the brain and the nervous system. It is one of the most destructive drugs in the world. Buprenorphine, has an agonist-antagonist receptor and is involved in the treatment of addiction with the effect on the mu-opioid receptor. cAMP response element-binding protein (CREB) is a cellular transcription factor. It binds to certain DNA sequences called cAMP response elements (CRE), thereby increasing or decreasing the transcription of the genes. and has been demonstrated to be involved in the neuronal adaptations that underlie drug related behaviors such as reward seeking and reduction of an individual s susceptibility to excessive drug intake. In this study, we investigated the effect of MA and buprenorphine on the expression levels of the CREB in the lumbar segment of the spinal cord of a male rats.Methods : 49 male Wistar rats were randomly assigned into seven experimental groups (n=7): Control, Saline, Methamphetamine (10 mg/kg, i.p. for 5 days), Buprenorphine (6 and 10 mg/kg, i.p.), Methamphetamine + Buprenorphine (6 and 10 mg/kg for 14 days). Lumbar spinal tissues were assayed for the expression of CREB gene using RT- PCR.Results : Results showed that chronic administration of methamphetamine increased the CREB gene expression in comparison to control group (p<0.01). Administration of 6mg/kg of buprenorphine did not change the expression level of CREB. Also, the expression of CREB gene was increased after the buprenorphine (10 mg/kg) administration (p<0.01). In other two methamphetamine addicted groups, administration of buprenorphine with two doses (6 and 10 mg/kg) for 14 days increased the level of CREB gene expression in comparison to methamphetamine or addicted groups (p> 0.05).Conclusion : It seems that CREB pathway plays a critical role in the development of methamphetamine toxicity. Buprenorphine by increasing the level of CREB gene expression in addicted group, may be involved in reducing of proinflammatory cytokines and has neuroprotective effects in treatment of psychostimulant drugs, also reduces feelings and interest in rewards from consumption

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نویسندگان

Seyed Hafez Hatami

Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran.

Homeira Hatami

Department of Animal Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran