Investigating Classes of Integrons among MDR Salmonella infantis Isolated from chicken broilers in the North of Iran
محل انتشار: پنجمین کنگره باکتری شناسی پزشکی ایران
سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 422
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شناسه ملی سند علمی:
MBMED05_065
تاریخ نمایه سازی: 1 دی 1397
چکیده مقاله:
Background: Salmonella is one of the most important food-borne pathogens and zoonotic agents all over the world. Antibiotic resistance is a growing problem in Salmonella isolates and spreading of resistance genes by integrons to susceptible strains is one of the major concerns in the emergence of resistant strains. The aim of the current study was the identification of classes of integrons among MDR Salmonella isolates frome chicken broilers in the North of Iran. Methods: In this study, all Salmonella isolates were obtained from the chicken broilers samples. The antibiotic susceptibility test was determined using the disk diffusion method agreeing with CLSI guideline. Cellular DNA was extracted by DNA Extraction Kit and PCR was performed for the identification of the intI, intII and intIII and resistance genes. Results: The molecular analysis of classes of integrons showed 36%, 42% and 4% isolates caring intI, intII and intIII genes, respectively.Out of the tetracyclines resistant isolates, 47(100%) and 5 (10.6%) carried tetA, tetB genes, respectively, while no isolate was positive for the tetG gene. Conclusions: The result of this study showed that integrons are common among MDR S. infantis and therefore can pose a serious risk to public health.
کلیدواژه ها:
نویسندگان
Zahra Moulana
Infectious Diseases and Tropical Medicine Research Center, Babol University of Medical Sciences, Babol, Iran.
Fariba Asgharpour
Faculty of Para-Medicine, Babol University of Medical Sciences, Babol, Iran.
Seyed Mahmoud Amin Marashi
Department of Microbiology and Immunology, Qazvin University of medical sciences, Qazvin, Iran