Deciphered from the Evolutionary Trend Analyses: Antibiotic Resistance Genes in Probiotics can be a Double Edged Sword

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 418

نسخه کامل این مقاله ارائه نشده است و در دسترس نمی باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

MBMED05_120

تاریخ نمایه سازی: 1 دی 1397

چکیده مقاله:

Background: Probiotics area group of health-promoting microorganisms, mainly fall in to lactic acid bacteria, which confer health benefits when used in an appropriate dosage. Considering their significant effect on the health parameters of the host, these beneficial microbes have been widely used in commercial food products and supplements. However, there are still some concerns since the recent studies have shed more light on the possible pathogenesis of these microbes in human and either farm animals. Such an alarming trait have been much more focused when a considerable range of antibiotic resistance genes were detected in probiotic genera. Hence, the evolutionary trend of the tetracycline resistance genes (tet), as an antibiotic resistance model, was analyzed to highlight its probable transferability and its possible role in the micro biome. Methods: The distribution of tet genes in the genus Lactobacillus was studied using the similarity searches in the BLASTN program which was followed by categorizing the origins of the isolates. Phylogenetic approaches were used to analyze the evolutionary relationship of tet genes in lactobacilli and either broader in Firmicutes. The target sequences derived from the nucleotide database of GenBank. The alignments were conducted based on the MAFFT program using the online CBRC and EMBL servers. Phylogenetic analyses were performed using the online Mr. Bayes program available at the CIPRES Science Gateway V. 3.3. Results: Similarity searches showed that a wide range of antibiotic resistance genes can be detected in a diverse array of Lactobacillus species. Interestingly, the presence of tet genes seems to be independent of the ecological niche of the species. Furthermore, such genes can be also found in commercial probiotic strains. Conclusion: The presence of antibiotic resistance genes in the probiotics is not always a risk factor although the transferability of such genes to opportunistic pathogens is significantly hazardous. A probable positive role of such antibiotic resistance genes in probiotics can be the reestablishment of the micro biome, in gut, vagina, etc, which is essential for health.

نویسندگان

Moslem Papizadeh

Microbiology Department, Pasteur Institute of Iran, Tehran

Mohammad Reza Pourshafie

Microbiology Department, Pasteur Institute of Iran, Tehran