SUB-LETHAL ANTIMICROBIAL PHOTODYNAMIC INACTIVATION AFFECTS QUORUM SENSING-CONTROLLED BIOFILM FORMATION GENE EXPRESSION OF PSEUDOMONAS AERUGINOSA IN-VITRO

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 440

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شناسه ملی سند علمی:

MEDISM19_001

تاریخ نمایه سازی: 13 مهر 1397

چکیده مقاله:

Background and Aim:The worldwide rise in antibiotic resistance made it mandatory to search for new alternative therapies that are able to eliminate resistant microorganisms and impair the development of new forms of resistance. In this context, antimicrobial photodynamic inactivation (APDI) is highlighted for the treatment of localized infections. During treatment, bacteria may expose to sub-lethal doses of APDI (sAPDI). Although sAPDI cannot kill microorganisms, it can significantly affect microbial virulence. In this study, we evaluated the effect of sAPDI using methylene blue (MB) on the expression of genes belonging to two quorum sensing (QS) operons (rhl and las systems) and two genes necessary for biofilm formation (pelF and pslA) under QS control in Pseudomonas aeruginosa ATCC 27853.Methods:Planktonic cells exposed to sAPDI (MB at 0.012 mM and red light dose of 23 J/cm2) were allowed to form biofilm for 24 h. Biofilm formation was evaluated using triphenyl tetrazolium chloride (TTC) assay and scanning electron microscopy (SEM). Gene expression of sAPDI-treated cells was evaluated by quantitative real-time polymerase chain reaction.Results:Quantitative assay (TTC) results and morphological observations (SEM) indicated that a single sAPDI treatment resulted in a significant decrease in biofilm formation ability of P. aeruginosa ATCC 27853 compared to their non-treated controls (P=0.012). sAPDI down-regulated the expression of QS-controlled biofilm formation genes (pslA and pelF) and QS genes (lasI, lasR, rhlI and rhlR) in P. aeruginosa ATCC 27853.Conclusion:Our results indicated that the transcriptional decreases caused by MB-sAPDI did lead to phenotypic changes.

کلیدواژه ها:

sub-lethal antimicrobial photodynamic inactivation ، biofilm formation ، quorum sensing ، Pseudomonas aeruginosa

نویسندگان

Saghar Hendiani

Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran

Nasim Kadhef

Department of Microbiology, School of Biology, College of Science, University of Tehran, Tehran, Iran

Majid Pornour

Photo Healing and Regeneration Research Group, Medical Laser Research Center, Academic Center for Education, Culture and Research (ACECR), Tehran, Iran