SEROTYPES OF LISTERIA MONOCYTOGENES ISOLATED FROM SPONTANEOUS HUMAN ABORTION IN TEHRAN,IRAN

Background and Aim:Listeria monocytogenes as a foodborne, sapronotic, zoonotic microorganism which is reported in many kinds of food stuffs, including, processed foods, milk and dairy products, vegetables, as well as row or ready- to-eat meat products. Surveys have indicated that Listeria monocytogenes may be present in approximately 4% of row milk samples examined in the USA. Listeria monocytogenes has also been recovered from unpasteurized dairy products.Methods:258 samples from 123 patients with spontaneous abortion were collected based on patient,s profile, age, second or third abortion trimester with biochemical and bacteriological methods, isolation comprised of 28 cases (18.8%) of Listeria monocytogenes from 118 (45.7%) placental bits, 53(20.5%) patient,s blood and 87(33.7%) from vaginal secretion. All samples were collected in complete sterilized condition and transformed into the laboratory on the same day of sampling. The samples after cold enrichment, were cultured on specific mediaResults:In this study 28(18.8%) Listeria monocytogenes with various serovars from 123 patients including 118(47.5%) placental bits, 54(20.5%) patients blood and 87(33.7%) vaginal swabs were isolatedConclusion:The correlation between the dominant serovars in spontaneous abortion in this research, indicated that total serovars with multiplex PCR method were confirmed, all serovars are kept in collection in order to detection of dominant genes, mostly hly and Iap .

کلیدواژه ها:

Humans ، Listeria monocytogenes ، spontaneous abortion ، dominant serovars