THE FREQUENCY AND VIRAL LOAD OF EPSTEIN-BARR VIRUS IN IRANIAN PATIENTS WITH HEMATOLOGIC MALIGNANCIES

Background and Aim:Epstein-Barr virus (EBV) has been associated with different malignancies, including Hodgkin lymphoma (HL), non-Hodgkin lymphoma (NHL) nasopharyngeal carcinoma and lymphoproliferative disorders. Patients with hematologic malignancies, chronic blood disorders could be infected with different types of this virus. This study reported the frequency and viral load of Epstein-Barr virus in Iranian patients with hematologic malignancies for estimation of possible factors affecting malignancy.Methods:Peripheral blood mononuclear cell (PBMC) of patients diagnosed with hematological malignancies obtained from individuals who referred to hospitals affiliated to Iran University of Medical Sciences, Tehran, Iran, from Sep 2016 to Sep 2017. A real-time PCR assay performed for detection and viral load evaluation of EBV by EBNA-3C gene. The quantitative real-time PCR SYBER Green method used by standard curve analysis. Standard was cloned into pTZ57R/T plasmid.Results:Of 79 subjects, 28 were NHL, 19 were HL, 20 were acute myeloblastic leukemia (AML), 11 were chronic lymphocytic leukemia (CLL), by the mean age ± Std. Deviation 59.64±19, 54±18.52, 55±18.72, 63.55±19.77, respectively. EBV EBNA-3C was detected in 43% (34/79) of all patients. The mean viral load ± std. deviation was 275148±1201944 and lower and higher viral loads were 280, 6622998 that detected in NHL group. Majority of EBV infection was in NHL group.Conclusion:there is not a clear relation between EBV infection and hematologic malignancies and EBV viral load cannot be significantly considered as one of the predisposing factors of hematologic malignancy in these patients. Further prospective studies by broader sample size and included interventions need to get comprehensive results.

کلیدواژه ها:

Epstein-Barr virus (EBV) ، hematologic malignancies ، EBNA3C ، tumorigenesis ، genotyping