FREQUENCY OF SEPSIS IN THE NEONATES AND DETERMINATION OF ANTIBIOTIC RESISTANCE OF ISOLATES IN ISFAHAN

Background and Aim:Sepsis is one of the serious infection in neonates. Prompt diagnosis and treatment play an important role in reducing neonatal mortality rate . The aim of this study was to determine the origin of this infection and antibacterial resistance pattern of the isolates.Methods:In this study 108 cases of Umbilical cord blood culture ، 153 peripheral blood culture were performed using BACTEC system. Bacterial isolates were identified by phenotypic and molecular methods. Antibiotic resistance patterns were determined using disk diffusion and Epsilometery methods . The PCR technique was used to determine the frequency of beta – lactamase genes.Results:Out of the 21 neonates with sepsis 6 were early – onset and 15 were late onset . Escherichia coli ، Staphylococcus epidermidis and Klebsiella pneumoniae were the most common isolates from peripheral blood culture. Escherichia coli and Stenotrophomonas maltophilia were isolated from two neonatal cord blood. Most resistance was to cefixime, ceftriaxone ، cefotaxime in Gram positive isolates and to ampicillin in Gram negative bacteria. The frequency of bla – OXA48 gene and bla- CTXM15 gene was 25 % and 50 %respectively.Conclusion:High prevalence of late – onset sepsis supports the role of the environment as a source of the neonatal sepsis . High drug resistance in the isolates reveals the importance of monitoring and accuracy in the use of antibiotics and improving infection control measures in the neonatal ward,

کلیدواژه ها:

Neonatal sepsis ، Antibiotic resistance ، Umbilical cord blood