DETECTION OF SGI1 AND ITS VARIANTS IN SALMONELLA SEROVARS ISOLATED FROM DIFFERENT HUMAN AND ANIMAL SOURCES

Background and Aim:The Salmonella genomic island 1 (SGI1) is a 42.4-kb mobilizable integrated element that contains several antibiotic resistance genes. The resistance genes are all located within the boundaries of a complex class 1 integron, which belongs to the In4 family. Variants of SGI1, containing different sets of resistance genes, have been found. SGI1, identified as a public health concern, is a horizontally transmissible genomic island. Lack of information exists on the presence of SGI1 variants among Salmonella isolates in Iran. This study was conducted to identify the MDR and SGI1 carrying Salmonella strains isolated from various sourcesMethods:A total of 242 Salmonella strains isolated from human, chicken, and cattle belonging to 11 serovars were studied. The strains were tested for their antimicrobial susceptibility and the prevalence of multidrug-resistance patterns. The isolates were investigated for the presence of SGI1 and its variants by PCR.Results:132 Salmonella isolates (54%) were resistant to at least one antibiotic, and more than 40% of the isolates were multidrug-resistant. Based on PCR, we distinguished 8 variants of Salmonella genomic island 1 including SGI1, SGI1-J, SGI1-F, SGI1-I, SGI1-O, SGI1-C, SGI1-B and SGI1-D in 102 human and animals isolates.Conclusion:This study showed the high prevalence of SGI1 and the variants (as the mechanism of multidrug resistance) in Salmonella serovars isolated from different animal and human sources. The high prevalence of this multidrug-resistance among Salmonella strains highlights the need for regulation of the use of antimicrobials in animals and humans, and to reduce the opportunity for organisms to develop resistance.

کلیدواژه ها:

SGI1 ، Salmonella ، Multidrug-resistance ، Animals ، Humans