FIRST REPORT OF SOME CLASS 1 INTEGRON-ASSOCIATED GENE CASSETTE ARRAYS AMONG ESCHERICHIA COLI AND KLEBSIELLA PNEUMONIAE ISOLATED FROM PATIENTS WITH URINARY TRACT INFECTION IN SOUTHWEST OF IRAN

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 328

نسخه کامل این مقاله ارائه نشده است و در دسترس نمی باشد

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این مقاله:

شناسه ملی سند علمی:

MEDISM19_172

تاریخ نمایه سازی: 13 مهر 1397

چکیده مقاله:

Background and Aim:In the current study,we aimed to determine the antibiotic resistance pattern, prevalence of classes 1-3 integrons and distribution of gene cassettes in Escherichia coli and Klebsiella pneumoniae isolated from patients with urinary tract infection (UTI)in southwest of Iran.Methods:Antibiotic susceptibility pattern of 340 clinical isolates of E. coli and K. pneumoniae were determined by Disk Agar Diffusion method. Polymerase chain reaction (PCR) was used for detection of class1, 2 and 3 Integrons. To analyze the gene cassettes, variable regions of class 1 integron were amplified by PCR and subjected to sequencing.Results:: Highest rates of resistance were observed to amoxicillin/clavulanic acid (38.8%) and cephalotin (32.1%) in K. pneumoniae and to amoxicillin/clavulanic acid (73.6%), trimethoprim-sulfamethoxazole (63.9%) and tetracycline (63.2%) in E. coli isolates. While Class 1 and class 2 integrons were identified in 62.5% and9% of E. coli isolates, only 12.2% of K. pneumoniae isolates were found to harbor the intI1 gene. Totally 10 and 7 different gene cassette arrays were found in theintI1 gene of E. coli and K. pneumoniae respectively. The dfrA17-aadA5 and aadA1 were the most prominent gene cassette arrays among E. coli and K. pneumoniae isolates respectively.Conclusion:Integrons played a more important role in the development of antibiotic resistance in E. coli compared to K. pneumoniae in our urinary isolates. For the first time we identified dfrA5-catB3-aacA4 andaadA1-blaOXA-30genecassette arrays in E. coli and also aadB-cat-blaOXA-10-aadAgene cassette array in K. pneumoniae isolates from Iran.

نویسندگان

Fariba Jahanbin

Department of Basic Sciences, Islamic Azad University, Yasooj Branch,Yasooj, Iran

Seyed Sajjad Khoramrooz

Cellular and Molecular Research Center, Yasuj University of Medical Sciences, Yasuj, Iran

Masoud Marashifard

School of veterinary Medicine, Shiraz University, Shiraz, Iran

Seyed Ali Asghar Malek Hosseini

Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran