FREQUENCY OF FIM GENE IN ESCHERICHIA COLI ISOLATES FROM URINARY TRACT INFECTIONS OF IMAM KHOMAINI HOSPITAL IN TEHRAN

سال انتشار: 1397
نوع سند: مقاله کنفرانسی
زبان: انگلیسی
مشاهده: 367

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شناسه ملی سند علمی:

MEDISM19_223

تاریخ نمایه سازی: 13 مهر 1397

چکیده مقاله:

Background and Aim:The most common etiologic factor of urinary tract infections (UTIs) is Escherichia coli (E.coli), accounting for 80% of these cases. The ability of the Uropathogenic Escherichia coli (UPEC) strains to cause the marked urinary tract infection depends on adhesion and binding of these strains to the urethral duct tissue cells made by fimbriate and non-fimbriate adhesions. Accordingly, in the present study one of the most well-known fimbrial adhesion in Escherichia coli type 1 fimbria expressed by fim gene was investigatedMethods:Urine samples were collected from patients referred or admitted to Imam Khomeini Hospital in Tehran in 2016 and 90 isolates of Escherichia coli were detected according to standard bacteriological tests. Then, the frequency of fim gene was evaluated by PCR method. E.coli ATCC25922 was used simultaneously.Results:All isolates with positive motility, negative urea, IMViC ++ - -, TSI A / A gas +, were confirmed as Escherichia coli. 70 of the 90 isolates (77.8% ) of Escherichia coli had fim gene by PCR method.Conclusion:According to the results, fim gene can be considered as one of the most common invasive factors of UPEC, which causes the adhesion of bacteria to the urethral duct tissue. And so the bacteria are not washed off by the flow of urine. In 22.2% of the investigated isolates of Escherichia coli, the fim gene was not detected, in which the infection could be due to other invasive factors.

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نویسندگان

Sepide Ghasemshahi

Department of Biology, College of science, Yadegar-e-Imam Khomeini(RAH) Shahr-e-Rey Branch, Islamic Azad University, Tehran, Iran

Maryam Bikhof Torbatil

Department of Biology, College of science, Yadegar-e-Imam Khomeini(RAH) Shahr-e-Rey Branch, Islamic Azad University, Tehran, Iran

Mojdeh Hakemi-Vala

Department of microbiology, Medical school, Shahid Beheshti University of Medical Sciences, Tehran, Iran