THE OCCURRENCE OF CTX-M-15 EXTENDED-SPECTRUM Β-LACTAMASE AMONG CLINICAL ISOLATES OF KLEBSIELLA PNEUMONIAE IN KHORRAMABAD, IRAN

Background and Aim:During the recent decade, CTX-M enzymes, mainly CTX-M-15 extended-spectrum β-lactamase (ESBL), have strikingly developed through the world. The objective of this study was to investigate the frequency of CTX-M-type β-lactamases and blaCTX-M-15 among Klebsiella pneumoniae isolated from hospitals in Khorramabad city.Methods:In this cross-sectional study, 60 isolates of K. pneumoniae were collected from selected teaching hospitals in Khorramabad, Iran. ESBL producing isolates were identified using phenotypic double-disk synergy test. The presence of CTX-M-types, as well as CTX-M-15 gene were investigated by PCR methodResults: While the highest resistance rates of isolates were found for nalidixic acid (65%) and trimethoprim/sulfamethoxazole (60%) antibiotics, the least resistance was to imipenem (15%). Moreover, 31(51.7%) isolates were resistant to at least three classes of antibiotics and designated as multidrug resistance (MDR). Fifty-two (86.7%) of 60 isolates, were ESBL positive. Thirty-five (58.3%) isolates harbored CTX-M-type β-lactamases, and also 29 (48.3%) isolates carried blaCTX-M-15.Conclusion:For the first time, our results prove the alarming dissemination of CTX-M-type β-lactamases, particularly CTX-M-15 in west of Iran. To better control of MDR K. pneumonia expansion, awareness and education of healthcare professionals as well as general population to true and limited using of antibiotics are urgently suggested.

کلیدواژه ها:

Extended-Spectrum β-lactamase ، Klebsiella pneumoniae ، CTX-M-15