: RELATIVE FREQUENCY BIOFILM FORMAITON STRAIN AMONG MULTI DRUG RESISTANCE PSEUDOMONAS AERUGINOSA, ISOLATED FROM PATIANT WITH BURN WOUND OF RASHT BURNE CENTER (VALAYET HOSPITA)

Background and Aim:Pseudomonas aeruginosa (pseudomonas aeruginosa) is one of the most important opportunistic pathogens in infections in patients hospitalized in different parts of hospitals, especially the burn. Various pathogenic factors, including secretion toxins on the one hand, and the increasing resistance of these bacteria to antibiotics, on the other hand, increase the importance of studying on strains isolated from patients. The purpose of this study was to simultaneously investigate the antibiotic resistance pattern and the presence of psla gene and biofilm production from pathogenicity indices in Pseudomonas aeruginosa. This study was performed on pseudomonads isolated from burn wounds of hospitalized patients at burn center of burn injury in Rasht provinceMethods:In this study 100 samples of Pseudomonas aeruginosa were isolated from hospitalized patients in Velayat Hospital of Rasht. Antibiotic resistance to 8 antibiotics (Gentamicin, Amikacin, Tobramycin, Ceftazidime, Piperacillin, Carbencililin, Ciprofloxacin and Imipenem) was assessed after confirmation of isolates and purification. Also, The production of biofilms and the presence of the psla gene were examined.Results:he antibiotic resistance levels were as follows: Carbenicilin (81%), Tobramycin (60%), Gentamicin (54%), Piperacillin (50%), Ciprofloxacin (49%), Stifaridium (25%), Amikacin (24%), (22%). Multidrug resistant isolates (MDR) (35%) and resistance to aminoglycosides (16%) were more biofilm generators than the antibiotics tested. (P = 0.001).Conclusion:The results of this study showed that AmiPenum was the most effective antibiotic and 100 (71%) strains produced biofilms, and 29 (29%) did not produce biofilms, and in 37 strains of production The psla gene was biofilm

کلیدواژه ها:

Pseudomonas aeruginosa ، PCR ، antibiotic resistance ، biofilm ، psla gene